Diapedesis

The article describes that diapedesis is the movement of white blood cells called leukocytes from circulating blood stream into areas of the body tissue where there is ongoing inflammatory reaction in response to cellular injury. The article evaluates the present facts on diapedesis and itemized several unresolved issues yet to be studied for perfect understanding of the biology of diapedesis. Though the author notes that compressive definition is poorly defined in scientific study, he however noted that diapedesis is technically accepted as transmigration of leukocyte between â€Å"endothelial cell-cell junctions† (Dejana, 106).Other upcoming research also has it that it can migrate through the endothelial cell. The articles points out literature reviews by several other authors to justify the possibility of transcellular and paracellular movements of leukocyte through the endothelial cells. The clarity from these two propositions is necessary to assist scientists in understa nding the arrangement and nature of proteins found along the pathway.This will help modulate the process and achieve desired aim(s) in clinical practice. The findings thus hold that leukocytes cross by biochemical interaction with molecular adhesive proteins at that line the endothelial junctions (Dejana 106). Some of the notable molecules are ICAM-1 and VCAM-1. Considering the intracellular movement, the article discussed that leukocyte can evade killing by the lysosome unlike some pathogen, to cross over into the tissue undergoing inflammation.Subsequent to the attachment of leukocyte to the ICAM-1 or VCAM-1, there is reshaping of the cytoskeleton of the cell to form transmissible cup like structure. The article raises several questions like what factors initiate the cup like formation, and which proteins play role in cytoskeletal conformational change. Answers to several questions the author raises are fundamental to scientific breakthrough in maneuvering process of inflammation to less harmful and more beneficial process in diseases e. g. cancer and organ infectious and toxic exposure. Reference Dejana Elisabetha. The Transcellular Railway: insights into leukocyte. Natural Cell Biology (2008) , Retrieved: April 24,2009. 105 – 107. Site Available at:

Biography of Dom Pedro I, First Emperor of Brazil

Biography of Dom Pedro I, First Emperor of Brazil Dom Pedro I (October 12, 1798–September 24, 1834) was the first Emperor of Brazil and was also Dom Pedro IV, King of Portugal. He is best remembered as the man who declared Brazil independent from Portugal in 1822. He set himself up as Emperor of Brazil but returned to Portugal to claim the crown after his father died, abdicating Brazil in favor of his young son Pedro II. He died young in 1834 at the age of 35. Fast Facts: Dom Pedro I Known For: Declaring Brazils independence and serving as emperorAlso Known As: Pedro de Alcà ¢ntara Francisco Antà ³nio Joo Carlos Xavier de Paula Miguel Rafael Joaquim Josà © Gonzaga Pascoal Cipriano Serafim, The Liberator, The Soldier KingBorn: October 12, 1798 in the  Queluz Royal Palace  near  Lisbon,  PortugalParents: Prince Dom Joo (later King Dom  Joo  VI), Doà ±a  Carlota JoaquinaDied: September 24, 1834  at Queluz Palace, Lisbon, PortugalAwards and Honors:  Multiple Brazilian and Portuguese titles and honorsSpouse(s): Maria Leopoldina, Amà ©lie of LeuchtenbergChildren: Maria (later Queen Dona  Maria  II of Portugal),  Miguel,  Joo,  Januria,  Paula,  Francisca, PedroNotable Quote: It grieves me to see my fellow humans giving a man tributes appropriate for the divinity, I know that my blood is the same color as that of the Negroes. Early Life Dom Pedro I was born with the lengthy name of Pedro de Alcà ¢ntara Francisco Antà ³nio Joo Carlos Xavier de Paula Miguel Rafael Joaquim Josà © Gonzaga Pascoal Cipriano Serafim on October 12, 1798, in the Queluz Royal Palace outside of Lisbon. He was descended from royal lineage on both sides: on his fathers side, he was of the House of Braganà §a, the royal house of Portugal, and his mother was Carlota of Spain, daughter of King Carlos IV. At the time of his birth, Portugal was ruled by Pedros grandmother Queen Maria I, whose sanity was quickly deteriorating. Pedros father Joo VI essentially ruled in his mothers name. Pedro became heir to the throne in 1801 when his older brother died. As a young prince, Pedro had the best schooling and tutoring available. Flight to Brazil In 1807, Napoleon’s troops conquered the Iberian Peninsula. Wishing to avoid the fate of the ruling family of Spain, who were â€Å"guests† of Napoleon, the Portuguese royal family and court fled to Brazil. Queen Maria, Prince Joo, young Pedro, and thousands of other nobles set sail in November of 1807 just ahead of Napoleon’s approaching troops. They were escorted by British warships, and Britain and Brazil would enjoy a special relationship for decades to follow. The royal convoy arrived in Brazil in January of 1808: Prince Joo set up a court-in-exile in Rio de Janeiro. Young Pedro rarely saw his parents; his father was very busy governing and left Pedro to his tutors and his mother was an unhappy woman who was estranged from her husband, had little desire to see her children, and lived in a different palace. Pedro was a bright young man who was good in his studies when he applied himself, but he lacked discipline. Pedro, Prince of Brazil As a young man, Pedro was handsome and energetic and fond of physical activities like horseback riding, at which he excelled. He had little patience for things that bored him, such as his studies or statecraft, although he did develop into a very skilled woodworker and musician. He was also fond of women and began a string of affairs at a young age. He was betrothed to Archduchess Maria Leopoldina, an Austrian Princess. Married by proxy, he was already her husband when he greeted her at the port of Rio de Janeiro six months later. Together they would have seven children. Leopoldina was much better at statecraft than Pedro and the people of Brazil loved her, although Pedro found her plain and continued to have regular affairs, much to Leopoldinas dismay. Pedro Becomes Emperor of Brazil In 1815, Napoleon was defeated and the Braganà §a family was once again rulers of Portugal. Queen Maria, by then long descended into madness, died in 1816, making Joo king of Portugal. Joo was reluctant to move the court back to Portugal, however, and ruled from Brazil via a proxy council. There was some talk of sending Pedro to Portugal to rule in his fathers place, but in the end Joo decided he had to go to Portugal himself in order to make sure that Portuguese liberals did not entirely do away with the position of the king and royal family. In April 1821, Joo departed, leaving Pedro in charge. He told Pedro that if Brazil started moving toward independence, he should not fight it and instead make sure he was crowned emperor. Independence of Brazil The people of Brazil, who had enjoyed the privilege of being the seat of royal authority, did not take well to returning to colony status. Pedro took his fathers advice, and also that of his wife, who wrote to him: The apple is ripe: pick it now, or it will rot. Pedro dramatically declared independence on September 7, 1822, in the city of So Paulo. He was crowned emperor of Brazil on December 1, 1822. Independence was achieved with very little bloodshed: some Portuguese loyalists fought in isolated locations, but by 1824 all of Brazil was unified with relatively little violence. In this, Scottish Admiral Lord Thomas Cochrane was invaluable: with a very small Brazilian fleet, he drove the Portuguese out of Brazilian waters with a combination of muscle and bluff. Pedro proved himself skillful in dealing with rebels and dissidents. By 1824, Brazil had its own Constitution and its independence was recognized by the United States and Great Britain. On August 25, 1825, Portugal formally recognized Brazils independence; it helped that Joo was the king of Portugal at the time. A Troubled Ruler After independence, Pedro’s lack of attention to his studies came back to haunt him. A series of crises made life difficult for the young ruler. Cisplatina, one of Brazil’s southern provinces, split off with encouragement from Argentina: it would eventually become Uruguay. He had a well-publicized falling-out with Josà © Bonifcio de Andrada, his chief minister and mentor. In 1826 his wife Leopoldina died, apparently of an infection brought on after a miscarriage. The people of Brazil loved her and lost respect for Pedro due to his well-known dalliances; some even said that she had died because he hit her. Back in Portugal, his father died in 1826 and pressure mounted on Pedro to go to Portugal to claim the throne there. Pedro’s plan was to marry his daughter Maria to his brother Miguel, which would make Maria queen and Miguel regent. The plan failed when Miguel seized power in 1828. Abdication of Pedro I of Brazil Pedro began looking to remarry, but word of his poor treatment of the respected Leopoldina preceded him and most European princesses wanted nothing to do with him. He eventually settled on Amà ©lie of Leuchtenberg. He treated Amà ©lie well, even banishing his longtime mistress, Domitila de Castro. Although he was quite liberal for his time- he favored the abolition of slavery and supported the Constitution- he continually fought with the Brazilian Liberal party. In March of 1831, Brazilian liberals and Portuguese royalists fought in the streets. He responded by firing his liberal cabinet, leading to outrage and calls for him to abdicate. He did so on April 7, abdicating in favor of his son Pedro, then 5 years old. Brazil would be ruled by regents until Pedro II came of age. Return to Europe Pedro I had great troubles in Portugal. His brother Miguel had usurped the throne and had a firm hold on power. Pedro spent time in France and Great Britain; both nations were supportive but unwilling to get involved in a Portuguese civil war. He entered the city of Porto in July of 1832 with an army consisting of liberals, Brazilians, and foreign volunteers. Things went poorly at first because King Manuels army was much larger and laid siege to Pedro in Porto for over a year. Pedro then sent some of his forces to attack the south of Portugal, a surprise move that worked. Lisbon fell in July 1833. Just as it looked like the war was over, Portugal got drawn into the First Carlist War in neighboring Spain; Pedros assistance kept Queen Isabella II of Spain in power. Death Pedro was at his best in times of crises, as the years of warring had actually brought out the best in him. He was a natural wartime leader who had a real connection to the soldiers and people who suffered in the conflict. He even fought in the battles. In 1834 he won the war: Miguel was exiled from Portugal forever and Pedros daughter Maria II was placed upon the throne. She would rule until 1853. The warring, however, took its toll on Pedros health. By September 1834, he was suffering from advanced tuberculosis. He died on September 24 at the age of 35. Legacy During his reign, Pedro I was unpopular with the people of Brazil, who resented his impulsiveness, lack of statecraft, and mistreatment of the beloved Leopoldina. Although he was quite liberal and favored a strong Constitution and the abolition of slavery, Brazilian liberals constantly criticized him. Today, however, Brazilians and Portuguese alike respect his memory. His stance on the abolition of slavery was ahead of its time. In 1972, his remains were returned to Brazil with great fanfare. In Portugal, he is respected for overthrowing his brother Miguel, who had put an end to modernizing reforms in favor of a strong monarchy. During Pedros day, Brazil was far from the united nation it is today. Most of the towns and cities were located along the coast and contact with the mostly unexplored interior was irregular. Even the coastal towns were fairly isolated from one another and correspondence often went first through Portugal. Powerful regional interests, such as coffee growers, miners, and sugarcane plantations were growing, threatening to split the country apart. Brazil could very easily have gone the way of the Republic of Central America or Gran Colombia and been split up, but Pedro I and his son Pedro II were firm in their determination to keep Brazil whole. Many modern Brazilians credit Pedro I with the unity they enjoy today. Sources Adams, Jerome R. Latin American Heroes: Liberators and Patriots from 1500 to the Present. New York: Ballantine Books, 1991.Herring, Hubert. A History of Latin America From the Beginnings to the Present.  New York: Alfred A. Knopf, 1962Levine, Robert M. The History of Brazil. New York: Palgrave Macmillan, 2003.

Specific Gravity - Definition of Specific Gravity

Specific Gravity - Definition of Specific Gravity The specific gravity of a substance is the ratio of its density to a specified reference substance. This ratio is a pure number, containing no units. If the specific gravity ratio for a given substance is less than 1, that means the material will float in the reference substance. When the specific gravity ratio for a given material is greater than 1, that means the material will sink in the reference substance. This is related to the concept of buoyancy. The iceberg floats in the ocean (as in the picture) because its specific gravity in reference to the water is less than 1. This rising vs. sinking phenomenon is the reason that the term specific gravity is applied, although gravity itself plays no significant role in this process. Even in a substantially different gravitational field, the density relationships would be unchanged. For this reason, it would be far better to apply the term relative density between two substances, but for historical reasons, the term specific gravity has stuck around. Specific Gravity for Fluids For fluids, the reference substance is usually the water, with a density of  1.00 x 103 kg/m3  at 4 degrees Celsius (waters densest temperature), used to determine whether or not the fluid will sink or float in water. In homework, this is usually assumed to be the reference substance when working with liquids. Specific Gravity for Gases For gases, the reference substance is usually normal air at room temperature, which has a density of approximately 1.20 kg/m3. In homework, if the reference substance is not specified for a specific gravity problem, it is usually safe to assume that you are using this as your reference substance. Equations for Specific Gravity The specific gravity (SG) is a ratio of the density of the substance of interest (Ï i) to the density of the reference substance (Ï r). (Note: The Greek symbol rho, Ï , is commonly used to represent density.) That can be determined using the following formula: SG Ï i à · Ï r Ï i / Ï r Now, considering that the density is calculated from mass and volume through the equation Ï  m/V, this means that if you took two substances of the same volume, the SG could be rewritten as a ratio of their individual masses: SG Ï i / Ï rSG mi/V / mr/VSG mi / mr And, since the weight W mg, that leads to a formula written as a ratio of weights: SG mi / mrSG mig / mrgSG Wi / Wr It is important to remember that this equation only works with our earlier assumption that the volume of the two substances is equal, so when we talk about the weights of the two substances in this last equation, it is the weight of equal volumes of the two substances. So if we wanted to find out the specific gravity of ethanol to water, and we know the weight of one gallon of water, then we would need to know the weight of one gallon of ethanol to complete the calculation. Or, alternately, if we knew the specific gravity of ethanol to water, and knew the weight of one gallon of water, we could use this last formula to find the weight of one gallon of ethanol. (And, knowing that, we could use it to find the weight of another volume of ethanol by converting. These are the sorts of tricks that you may well find among homework problems that incorporate these concepts.) Applications of Specific Gravity Specific gravity is a concept that shows up in a variety of industrial applications, particularly as it relates to fluid dynamics. For example, if youve ever taken your car in for service and the mechanic showed you how small plastic balls floated in your transmission fluid, youve seen specific gravity in action. Depending on the specific application in question, those industries may use the concept with a different reference substance than water or air. The earlier assumptions applied only to homework. When you are working on a real project, you should know for sure what your specific gravity is in reference to, and shouldnt have to make assumptions about it.

The American Dream Essay Example | Topics and Well Written Essays - 750 words

The American Dream - Essay Example Far from being dead, the American dream is alive and kicking. For many people, home-ownership has been a large part of the American dream. Owning your own house means controlling your own destiny and having a large asset to borrow against. For many years, the government encouraged this idea and provided tax breaks and other incentives to ensure that people would be able to buy their own homes. While some suggest that these policies helped to create a housing bubble, this does not mean the dream is wrong. The problem begins when a dream is subsidized by the government. Governments are not responsible for giving us our dreams. They should move out of the way and let people be free to pursue their own ideas. Rather than killing the American dream, the governments program of subsidizing home ownership effectively distorted the dream out of recognition. Now it is back again as the economy recovers. Furthermore, the dream is expanding around the world. In Russia, almost 70 per cent of peop le live in apartment buildings and do not own their own homes. The Russian government wants to encourage more home ownership and copy this element of the American dream (Ustinova). There are other elements of the American dream which play an important role in the idea that the dream is alive and kicking. No one can dispute that America has had problems with racial issues in the past. Until the Civil War and emancipation, it can be argued that there was no such thing as the American dream, that it simply did not exist because a large number of Americans were enslaved. Following Reconstruction there was still a lot of racial problems and schools in the South were not desegregated until the 1950s. Perhaps then the dream really came to life as it became at least possible for African-Americans to achieve success in America. But in recent years this dream has come thrillingly to life. With the election of Barack Obama in 2008, America showed that the dream is more alive than ever. Indeed the long-standing American dream of upward mobility and fairness for all was wedded powerfully to Martin Luther King Jr.'s dream of racial equality. For the first time a black man was in the White House. Anything was possible. Some people dispute this and suggest that President Obama's father was African and not African-American and therefore did not have to fight the civil rights battles, but could simply walk into an American university unopposed. But this idea overlooks the true power of the American dream. If the American dream were dead, why would so many people wish to live in America. Why would America continue to be a beacon of hope and prosperity to the rest of the world. Americans live some of the most comfortable lives in the world (Ownby). Consumer and luxury goods are plentiful and available for excellent prices everywhere. Life for most Americans is good and getting better. Unemployment issues will soon be resolved. America is on path to make a more perfect union, welc oming new immigrants to share in their prosperity. Recent problems will not last. The dream is alive and well. It is so strong is cannot be knocked off course by a minor financial crisis. The truth is that America is not in decline. Its system is working fine and the current problems are just a blip on the radar. American is a powerful country that believes in freedom. Its home ownership market is improving and the economy is recovering. Minorities have never had as many opportunities as they do today and Barack Obama is President.

VOTING Essay Example | Topics and Well Written Essays - 750 words

VOTING - Essay Example According Lijphart (1999, p69), Cleisthenes introduced one of the earliest recorded systems of democracy in Greece back in 508 BC. This system of democracy required voters to select the least preferred politician who was then exiled for ten years in a distant country. The minimum number of votes that were required to have a politician exiled was 6000.However, if more than one politician received more than the set threshold votes, the candidate with the higher number of votes was exiled instead( Lijphart, 1999,p72). In the thirteenth century, the Venetian state created one of the most developed electoral systems at the time. The Venetian system nominated forty members in ‘Great Council’ and in the mid 13 century, the number of council members elected was increased to sixty (Grofman, & Merrill, 1999, p53) Though Venetians applied different electoral systems, approval-voting system was the most popular. The approval system required voters to cast a single vote for every can didate vying for a position in the council. Eventually, the candidate who garnered the highest number of votes was declared the winner to represent the state in the council (Grofman, & Merrill, 1999, p38). ... Equally important all candidates should be treated equally, without favoring a particular person over another. By treating the candidates equally, Schofield (2001, p584) notes that the voters are presented with a favorable opportunity of electing a person of their choice without bias and prejudice of any sort. Fair elections should also ensure that the victory goes to the candidate with the highest number of votes. Different governments and organizations apply various methods of ensuring effectiveness of the participants’ votes. According to Lijphart (1999, p102), votes become ineffective in electoral processes that lack fairness and transparency to both voters and the candidates. These malpractices include allowing particular voters to cast more than one vote, discriminating voters based on their gender, social and economic status, tribe, or ethnicity. In such cases, the votes become ineffective because people are not given an opportunity to elect candidates of their choice ( Lijphart, 1999, p105). In United states for instance, debate on whether setting the minimum voting age amounts to discrimination of young voters still persist. Candidates should be given an equal platform for presenting their candidature to the electorate. However, Schofield (2001, p587) notes that many electoral systems in the world contain elements of unfairness that favor a particular candidate over another or others. In such cases, the preferred candidate receives preferential treatment in form of positive media coverage, favorable popularity ratings among other practices at the expense of the other candidates. These practices make the vote to be ineffective because the

Ballistics Research Paper Example | Topics and Well Written Essays - 2000 words

Ballistics - Research Paper Example o empirical evidence to back up this claim that criminal cases have been affected because the jurors are now more well-versed in Forensic Science through these television series—making it more difficult to get a guilty verdict as jurors require more forensic evidence—a study has been conducted on 1027 jurors that show that â€Å"46 % expected to see some kind of scientific evidence in every criminal case† (Barak, Kim and Shelton, 2006 as cited in Shelton, 2008, p. 5). Now, because gun-related crimes continue to rise since 2002, with 68% of murders in the United States in the year 2006 being committed with the use of a firearm, it can be said that much of the scientific evidence being expected by jurors relate to ballistics, which is a specialized area in the field of Forensics (National Institute of Justice, 2010, para. 2). In fact, the study on â€Å"the CSI Effect† found that â€Å"32 % expected to see ballistic/firearms laboratory evidence in every criminal case† (p. 5). This introduction into Forensic Science and Ballistics does not only highlight their key role in crime-solving, but also emphasizes the role of scientific evidence in serving justice. It can be said, then, that no matter how the television series may have exaggerated some aspects of crime scene investigation, like the time the evidence is processed, it cannot be refuted that the field of Forensics and Ballistics play crucial roles in the criminal justice systemâ €”CSI Effect or not. Having established the importance of Forensic Science and Ballistics, I will now outline what this paper will contain. In the course of my paper I will provide background and history of the field and I will then examine the many changes undergone by ballistics in the 20th century. Case studies will be examined to highlight the points that I will be making. I will then conclude with some thoughts as to where the field may go in the future. Forensic Science, most commonly known by its shortened name of Forensics, is

Challenges Faced By Medical Laboratories Information Technology Essay

Challenges Faced By Medical Laboratories Information Technology Essay Healthcare is always a top priority within any collection of individuals, whether it is the workforce, schools, churches, communities, states, countries etc. This is because mortality rates are directly affected by the level of healthcare available, and effective productivity is dependent on good health and high mortality rates. Developed nations tend to invest large sums of money into healthcare, in order to prevent most ailments before they become epidemics, however, due to lack of emphasis on the importance of healthcare, African nations suffer from low mortality rates caused by lack of proper laboratory procedures and equipment, to test and accurately diagnose problems ahead of time. Even though we are now in a highly advanced technological era, Nigerian hospitals still conduct tests, data collection, and calculations manually, leaving a huge opening for errors which could lead to wrong diagnosis and in turn, wrong treatment. As we humans become ever increasingly dependent on computer technology in our daily lives, it then would be appropriate to utilize technological solutions to problems conventional methods prove inefficient at. These problems can be solved with the use of a properly configured software system to manage all administrative tasks in the laboratories. The most effective use of technology to solve to these issues would be carried out using open source software popularly known as a Laboratory Information System (L.I.M.S). The model of this work seeks to provide an application which will enable vital functionalities such as proper documentation and storage of patient information, patient specimen/sample tracking, and most important, patient test results. In the light of the above, the proposed system applies the web application development approach in its information architecture and processing, however this system will run on a local machine as opposed to running on a remote server over the internet. Consequently at completion of this work, the end product should be a Laboratory information management system which handles activities in the lab from the entry of a patient to the laboratory to the generating of a test result or Laboratory report. BACKGROUND OF STUDY The study for the development of the laboratory information management system uses a medical laboratory facility: Bakor Medical laboratory as its case study. Investigations indicate that the following steps or procedures are undertaken in the process of getting tested in the medical laboratory. On entry into the laboratory a document known as the Patient Investigation form. This form holds information such as the Patients name, age sex, on filling this form the patient is then billed. The patient Investigation form is then transferred to a second laboratory attendant who then uses the information retrieved from the aforementioned form to fill the laboratory request book. STATEMENT OF THE PROBLEM In recent times, due to increase in population, there is constant pressure on providers of various services to provide innovative methods of dispensing a sizable amount of services to great amount of people in the shortest possible time. Consequently, organizations are constantly resorting to technological solutions to meet up with the ever increasing demand for quality and speedy service delivery and with virtually everything in our day to day lives being technologically driven, should there not be a scenario where paper documentation is completely annihilated from medical laboratories?, where a system is adopted in which patient records and data are properly stored such as to enable features such as patient/visitor history tracker, where a returning patients history can aid the laboratory in deducing what type of test a visitor would request for, statistical reports generation where useful statistical information is inferred based on test results e.g whether or not there is an incr ease or decrease of new HIV infections, should there not be a software/application where other stakeholders in the health-care delivery processes such as doctors and pharmacists have access to laboratory generated information to aid in their health-care administration?, should the retrieval of patient test records be slow and cumbersome?, why must results be entered directly on the result document, therefore a backup copy is unavailable. Questions such as these will serve as a guide to the development of a robust system than manages various tasks in the medical laboratory. 1.3 RESEARCH QUESTIONS Based on the statement of the problems above the research question for this study are: How will the development of a laboratory information management System greatly increase the laboratorys efficiency? OBJECTIVE OF STUDY To design a robust Laboratory information System that will efficiently aid in the running of the laboratory facility To hold and provide timely information about each visitor to the laboratory To reduce the need to hire staff through the proper application of technological solutions therefore cutting cost. With a proper functioning and comprehensively designed application, Laboratory tasks such the following can be achieved: SAMPLES MANAGEMENT A LIMS can automate the management of samples. An organization can configure its analytical parameters and calculations into the LIMS before implementing the software in the laboratory. After sample registration, the system can print barcodes which it can scan at the end of the analysis when loading results into the LIMS. The system can check the completed results, automatically validating those which comply with specifications; and reporting (but not validating) out-of-specification results. A LIMS may release or retain lots and batches, according to a laboratorys specifications and calculations. Once results come available for the labs clients or owners, they can extract them in  PDF, XML  or spreadsheet files from the LIMS interface. (Note that moving insufficient data to a spreadsheet may lose the traceability of changes). LABORATORY USERS One may configure a LIMS for use by an unlimited  number of users. Each user owns an interface, protected by security mechanisms such as a login and a password. Users may have customized interfaces. A laboratory manager might have full access to all of a LIMS functions, whereas technicians might have access only to functionality needed for their individual work-tasks. ADMINISTRATIVE TASKS AUTOMATION As of 2009  LIMS implementations can manage laboratory sampling, consumables sampling schedule and financial (invoices). SCOPE OF STUDY Bakor Medical centre has various departments and sections based on the issue at hand to be dealt with; however this study focuses mainly on the lab processes, including data collection and management. This study takes the patient from the moment of entry into the medical centre, filling forms, up till the moment the test is taken, from that point onwards the study will focus on the methods used by the laboratory attendant to collect and store data, through recording of findings and submission of results This study will also concentrate on data backup and retrieval methods and will highlight potential errors and problems that could be encountered if the entire process was carried out manually instead of using a well configured computer application. SIGNIFICANCE OF STUDY The Computer based Laboratory information management System is quite desirable as it will benefit the laboratory in the area of repetitive task automation. It will serve as an assistant to the medical laboratory scientist. It will also benefit patients as they will be able to retrieve record from previous visits to the laboratory. LIMITATIONS OF STUDY The limitations of this study include: Inability to obtain actual test result document, as this goes against medical ethics for a third party to view Laboratory specimen or test results. Unavailability of qualified laboratory scientist at the study locations to properly explain terms and laboratory procedures. LITERATURE REVIEW From tasting urine to microscopy to molecular testing, the sophistication of diagnostic techniques has come a long way and continues to develop at breakneck speed. The history of the laboratory is the story of medicines evolution from empirical to experimental techniques and proves that the clinical lab is the true source of medical authority. Three distinct periods in the history of medicine are associated with three different places and therefore different methods of determining diagnosis: From the middle ages to the 18th century, bedside medicine was prevalent; then between 1794 and 1848 came hospital medicine; and from that time forward, laboratory medicine has served as medicines lodestar. The laboratorys contribution to modern medicine has only recently been recognized by historians as something more than the addition of another resource to medical science and is now being appreciated as the seat of medicine, where clinicians account for what they observe in their patients. The first medical diagnoses made by humans were based on what ancient physicians could observe with their eyes and ears, which sometimes also included the examination of human specimens. The ancient Greeks attributed all disease to disorders of bodily fluids called humors, and during the late medieval period, doctors routinely performed uroscopy. Later, the microscope revealed not only the cellular structure of human tissue, but also the organisms that cause disease. More sophisticated diagnostic tools and techniques such as the thermometer for measuring temperature and the stethoscope for measuring heart rate were not in widespread use until the end of the 19th century. The clinical laboratory would not become a standard fixture of medicine until the beginning of the 20th century. Ancient diagnostic methods In ancient Egypt and Mesopotamia, the earliest physicians made diagnoses and recommended treatments based primarily on observation of clinical symptoms. Palpation and auscultation were also used. Physicians were able to describe dysfunctions of the digestive tract, heart and circulation, the liver and spleen, and menstrual disturbances; unfortunately, this empiric medicine was reserved for royalty and the wealthy. Other less-than-scientific methods of diagnosis used in treating the middle and lower classes included divination through ritual sacrifice to predict the outcome of illness. Usually a sheep would be killed before the statue of a god. Its liver was examined for malformations or peculiarities; the shape of the lobes and the orientation of the common duct were then used to predict the fate of the patient. Ancient physicians also began the practice of examining patient specimens. The oldest known test on body fluids was done on urine in ancient times (before 400 BC). Urine was poured on the ground and observed to see whether it attracted insects. If it did, patients were diagnosed with boils. The ancient Greeks also saw the value in examining body fluids to predict disease. At around 300 BC, Hippocrates promoted the use of the mind and senses as diagnostic tools, a principle that played a large part in his reputation as the Father of Medicine. The central Hippocratic doctrine of humoral pathology attributed all disease to disorders of fluids of the body. To obtain a clear picture of disease, Hippocrates advocated a diagnostic protocol that included tasting the patients urine, listening to the lungs, and observing skin color and other outward appearances. Beyond that, the physician was to understand the patient as an individual. Hippocrates related the appearance of bubbles on the surface of urine specimens to kidney disease and chronic illness. He also related certain urine sediments and blood and pus in urine to disease. The first description of hematuria or the presence of blood in urine, by Rufus of Ephesus surfaced at around AD 50 and was attributed to the failure of kidneys to function properly in filtering the blood. Later (c. AD 180), Galen (AD 131-201), who is recognized as the founder of experimental physiology, created a system of pathology that combined Hippocrates humoral theories with the Pythagorean theory, which held that the four elements (earth, air, fire, and water), corresponded to various combinations of the physiologic qualifies of dry, cold, hot, and moist. These combinations of physiologic characteristics corresponded roughly to the four humors of the human body: hot moist = blood; hot dry = yellow bile; cold moist = phlegm; and cold dry = black bile. Galen was known for explaining everything in light of his theory and for having an explanation for everything. He also described diabetes as diarrhea of urine and noted the normal relationship between fluid intake and urine volume. His unwavering belief in his own infallibility appealed to complacency and reverence for authority. That dogmatism essentially brought innovation and discovery in European medicine to a standstill for nea rly 14 centuries. Anything relating to anatomy, physiology, and disease was simply referred back to Galen as the final authority from whom there could be no appeal. Middle Ages In medieval Europe, early Christians believed that disease was either punishment for sin or the result of witchcraft or possession. Diagnosis was superfluous. The basic therapy was prayer, penitence, and invocation of saints. Lay medicine based diagnosis on symptoms, examination, pulse, palpitation, percussion, and inspection of excreta and sometimes semen. Diagnosis by water casting (uroscopy) was practiced, and the urine flask became the emblem of medieval medicine. By AD 900, Isaac Judaeus, a Jewish physician and philosopher, had devised guidelines for the use of urine as a diagnostic aid; and under the Jerusalem Code of 1090, failure to examine the urine exposed a physician to public beatings. Patients carried their urine to physicians in decorative flasks cradled in wicker baskets, and because urine could be shipped, diagnosis at long distance was common. The first book detailing the color, density, quality, and sediment found in urine was written around this time, as well. By a round AD 1300, uroscopy became so widespread that it was at the point of near universality in European medicine. Consequently, the clinical laboratory became a standard fixture of medicine at the beginning of the 20th century; it is now an integral part of the health-care delivery process and is seen as the basis for medical diagnosis. In recent times a medical laboratory scientist (MLS), formerly known as a medical technologist (MT) or clinical laboratory scientist (CLS), functions as a medical detective, performing laboratory tests that provide physicians with information that assists them in preventing, diagnosing and treating diseases and maintaining patient wellness.   The medical laboratory scientist performs a wide variety of laboratory tests, ranging from simple dipstick urine tests to complex DNA tests that help physicians assess risk of diseases. Using test results, physicians can uncover diabetes, cancer, heart attacks, infections and many other diseases.   Medical laboratory scientists interact with physicians, nurses, pharmacists, and other members of the healthcare team to provide timely, accurate information so the patient can receive the correct medical treatment.   Medical laboratory scientists use sophisticated biomedical instruments and technology, microscopes, complex electronic equipment, computers, and methods requiring manual dexterity to perform tests on blood, body fluids, and tissue specimens. Clinical laboratory testing sections include clinical chemistry, hematology, Immunohematology (Blood Bank), immunology, microbiology and molecular diagnostics. EFFECTS OF MEDICAL LABORATORIES Medical Laboratories have played a pivotal role over the years. As stated above, in ancient times, physicians relied on various inaccurate means of disease diagnosis such as urine tasting, listening to the lungs etc. However with the advent and subsequent evolution of modern medical laboratory facilities, plus the discovery of the cellular nature of human tissue and the invention of the microscope, medical diagnosis made a tremendous leap from a 50-50 accuracy ratio to an 80% accuracy rating for laboratory based medical diagnosis. Therefore the use of medical laboratories has greatly increased the accuracy of diagnosis; hence the physician can administer the proper kind of treatment. The use of laboratories has also led to various discoveries, such as new strands of Viruses, bacteria, parasites and fungi. It also acts as a monitor for new strands of drug resistant bacteria. CHALLENGES FACED BY MEDICAL LABORATORIES The main challenges and bottle-necks encountered by medical laboratories over the years include. Inability to preserve patient samples or specimens such as blood, sputum, stool, over a long period of time for reference purposes hence making patient to specimen matching and tracking, Efficient information sharing and retrieval between the laboratory scientist and those administering treatment has been a problem. MEDICAL LABORATORIES: IMPROVEMENTS AND THE FUTURE For the efficient functioning of the diagnosis system, health-care delivery, scientific research into bacteriology and disease causing organisms, technology should be applied to automate administrative tasks, such as the visitor registration and result documentation. By making judicious use of computer software to automate and manage tasks in the laboratory there will be a dramatic increase in its efficiency. Consequently this will greatly reduce the need to recruit and pay personnel to carry out administrative duties therefore such resources can be channeled towards more important needs of the laboratory. TECHNOLOGY PLATFORM/PROGRAMMING LANGUAGE TO BE USED IN STUDY The technologies to be used in the development of this Laboratory Information Management system is an open source programming language known as PHP, together with a MYSQL driven database, a solid browser based application will be developed. PHP: PHP also known as Hypertext Pre-processor, it belongs to a class of languages known as middleware (Needham, 2006). These languages work closely with the web server to interpret the request made from the web, processes these request, interact with other programs on the server to fulfill the request and then indicates to the web server exactly what to serve to the clients browser. It is the leading web programming language for design of web applications. It possesses a language similar to C, Java or Pearl. Its uses include: retrieving user input and saving it in a database, retrieving information from a database and general data manipulation processes. THE CLIENT: Simply refers to end users of an application that connect to a remote server to carry out computational processes THE SERVER: An application known as a web server listens for requests a client makes, responds to those requests and serves out the appropriate response (Greenspan, 2002) MYSQL: Refers to an open source relational database management system with a set of programs that access and manipulate these records. (Descartes, 2003). It is a  relational database management system (RDBMS)  that runs as a server providing multi-user access to a number of databases. APACHE WEB SERVER: Apache is a web server notable for playing an important role in the initial growth of the world-wide web. It is responsible for accepting Hypertext Transfer Protocol (HTTP) request from web client (web browsers) and serving the HTTP responses along with optional data content which usually are HTML pages. These platforms are used in this research work because: There are open source meaning there are free to use and develop with without having to purchase licenses or fulfill any legal obligation to the owners of the technology. It is easy to deploy on a local machine Its hardware requirements are minimal therefore making its installation and usage less cumbersome. Developer tools and support services for the platform/technology are readily available at absolutely no cost. DATA COLLECTED FOR LABORATORY TEST Lab Investigation Form: This form is used for general patient registration and data collection. Data collected on this form include Patient Name Patient age Hospital Number Specimen Blood Specimen Sputum Stool Urea Various Swap Investigation Record Clinic Details Name of Doctor Lab Request Book: Used to record Patients data for that day. Name Lab Investigation Done Amount Time in Data Collected for lab tests Lab result book (Used for recording patients results) Patients Name Date of birth Gender Test Results Date Hospital registration Number Extra Comments SUMMARY The use of technological solutions greatly reduces costs, increase profits, save human effort and provide better services to customers/clients The use of an LIS (Laboratory information System) in the running of a medical laboratory facility greatly improves the documentation process; makes patients records retrieval a lot easier and faster, records are not lost and are kept safe via regular backup of the available data. With the use of a Laboratory information system, various kinds of data deductions, surveys and reports can be easily generated for statistical purposes such as the average percentage of persons with a certain kind of disease, Genotype or blood type etc. CONCLUSION A properly developed Medical Laboratory information system will greatly increase productivity, increase the quality of services delivered by the facility and greatly reduce the amount of man hours put into the delivering the laboratory services